Search results for "séquençage haut débit"

showing 3 items of 3 documents

Regional-scale analysis of arbuscular mycorrhizal fungi: the case of Burgundy vineyards

2016

SPE IPM INRA UB; Aim : To improve knowledge of arbuscular fungal communities for a sustainable management in vineyards. Methods and results : In 16 plots across Burgundy under contrasted soil properties and agricultural practices, we assessed arbuscular mycorrhizal fungal (AMF) diversity in vine roots, using pyrosequencing of ribosomal Internal Transcribed Spacers (ITS). AMF sequences could be retrieved from all plots across Burgundy, both in organic and in conventional vineyards with high chemical inputs. Sequences from the survey were almost exclusively affiliated to molecular taxa in the Glomerales, including six “core species” found in all plots, corresponding to 77% of all sequences, s…

0106 biological sciencesagroecologyVine[SDV]Life Sciences [q-bio]agroécologieHorticultureBiology01 natural scienceslcsh:Agriculturelcsh:BotanyBotanyBourgogne[SDV.BV]Life Sciences [q-bio]/Vegetal Biologymycorhizes à arbusculesAgroecologyespaceurs internes transcrits (ITS)business.industryarbuscular mycorrhizafungilcsh:SSpecies diversityhigh-throughput sequencinginternal transcribed spacers (ITS)04 agricultural and veterinary sciencesbiology.organism_classificationlcsh:QK1-989Arbuscular mycorrhizaséquençage haut débitTaxonAgriculture[SDE]Environmental Sciences040103 agronomy & agriculture0401 agriculture forestry and fisheriesPyrosequencingGlomeralesbusinessBurgundy010606 plant biology & botanyFood ScienceOENO One
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Changements à court terme de la composition des communautés de champignons mycorhiziens à arbuscules et des populations de Rhizophagus irregularis en…

2014

La mycorhize à arbuscule a un important potentiel d’amélioration de la nutrition minérale des plantes, fort utile dans le contexte agricole. Cependant, certaines pratiques culturales sont connues pour avoir un effet néfaste sur la diversité des champignons mycorhiziens à arbuscules (Glomeromycètes). Il est donc nécessaire de mieux comprendre les dynamiques de leurs communautés et populations pour pouvoir préserver et gérer le potentiel symbiotique. Dans cette étude, nous avons étudié les effets séparés et conjoints du retournement du sol et des fertilisations phosphatée et azotée du sol sur la structure des communautés des Glomeromycètes et sur les populations de l’espèce Rhizophagus irregu…

[SDE] Environmental Sciences[SDV]Life Sciences [q-bio]Glomeromycètespopulationscommunautéfertilisation[SDV] Life Sciences [q-bio]rpb1séquençage haut débit[SDE]Environmental Sciences[SDV.BV]Life Sciences [q-bio]/Vegetal Biology[SDV.BV] Life Sciences [q-bio]/Vegetal BiologyRFLPmtLSUbande enherbéeRhizophagus irregularispratique culturales
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Genomic characterization of mosaic cutaneous pigmentary disorders

2019

Ntroduction: Mosaic cutaneous dyschromia is strongly evocative of an underlying genetic mosaicism. These post-zygotic events are challenging for conventional diagnostic tools. Thus, genetic basis of mosaic cutaneous dyschromia still remained poorly understood. Materials and Methods: The M.U.S.T.A.R.D. cohort gathers DNA from skin biopsies of patients with mosaic cutaneous dyschromia. After a specialised phenotype analysis, they are referred to either trio exome sequencing (ES) at 200X, or targeted ultra-deep sequencing (60,000X) of candidate genes. Data are analysed with a tailored pipeline, allowing detection of both low-rate nucleotidic variations or chromosomal events. Results: From 2013…

[SDV.MHEP] Life Sciences [q-bio]/Human health and pathologySéquençage haut débitNext generation sequencingMosaïqueCutaneous pigmentationHypomelanosis of ItoPigmentation cutanéeMosaic[SDV.MHEP]Life Sciences [q-bio]/Human health and pathologyHypomélanose d'Ito
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